AML Panel: FLT3 & NPM1 Mutation Detection by PCR
Test Code: AMLP
Description of Testing:
Primers targeting the area surrounding the ITD and D835 regions of the FLT3 gene and exon 12 of the NPM1 gene are used to amplify the patientís DNA. The size of the ITD and NPM1 PCR product is determined by capillary electrophoresis. The D835 PCR product is digested with EcoRV and the presence of the mutation is assessed using capillary electrophoresis.
Indications for Testing:
- Stratifying high and low risk AML
- At initial diagnosis of AML
- Recurrence of leukemia after induction therapy on patients not initially screened for FLT3 or NPM1 mutations.
Turn-Around Time:
3-5 Days
CPT Codes:
83891 - Isolation or extraction of highly purified nucleic acid
83898x3 - Amplification of patient nucleic acid, single primer pair, each primer pair
83892 - Enzymatic digestion
83909x3 - Separation and identification by capillary electrophoresis
83912 - Interpretation and report
Storage Conditions:
Room Temp up to 72 hours
4°C up to 7 days
Shipping Conditions:
Ambient or Cool; Do not freeze
Specimen Requirements:
5 ml of Peripheral Blood in EDTA, ACD, or Heparin
3 ml of bone marrow in EDTA, ACD, or Heparin
1 ug of previously isolated DNA
