FLT3 Mutation Detection by PCR
Test Code: FLT3
Description of Testing:
The ITD and D835 containing regions of the FLT3 gene are amplified. The size of the ITD PCR product is determined by capillary electrophoresis. The D835 PCR product is digested with EcoRV and the presence of the mutation is determined by capillary electrophoresis.
Indications for Testing:
- Stratifying high and low risk AML
- At initial diagnosis of AML, ALL and MDS
- Recurrence of leukemia after induction therapy on patients not initially screened for FLT3 mutations.
Turn-Around Time: 3-5 Days
CPT Codes:
83891 - Isolation or extraction of highly purified nucleic acid
83898x2 - Amplification of patient nucleic acid, single primer pair, each primer pair
83892 - Enzymatic digestion
83909x2 - Separation and identification by capillary electrophoresis
83912 - Interpretation and report
Storage Conditions:
Room Temp up to 72 hours
4°C up to 7 days
Shipping Conditions:
Ambient or Cool; Do not freeze
Specimen Requirements:
5 ml of Peripheral Blood in EDTA, ACD, or Heparin
3 ml of bone marrow in EDTA, ACD, or Heparin
1 ug of previously isolated DNA
FLT3 Mutation Testing is performed pursuant to patents licensed from Takara Bio of Otsu, Japan.
