NPM1 Mutation Detection by PCR
Test Code: NPM1
Description of Testing:
Primers targeting exon 12 on the NPM1 gene are used to amplify the patient's DNA. The size of the NPM1 PCR product is determined by capillary electrophoresis.
Indications for Testing:
- Stratifying high and low risk AML
- At initial diagnosis of AML
- Recurrence of leukemia after induction therapy on patients not initially screened for NPM1 mutations.
Turn-Around Time: 3-5 Days
CPT Codes:
83891 - Isolation or extraction of highly purified nucleic acid
83898 - Amplification of patient nucleic acid, single primer pair, each primer pair
83909 - Separation and identification by capillary electrophoresis
83912 - Interpretation and report
Storage Conditions:
Room Temp up to 72 hours
4°C up to 7 days
Shipping Conditions:
Ambient or Cool; Do not freeze
Specimen Requirements:
5 ml of Peripheral Blood in EDTA, ACD, or Heparin
3 ml of bone marrow in EDTA, ACD, or Heparin
1 ug of previously isolated DNA
NPM1 Mutation Testing is performed pursuant to patents licensed from TrovaGene, Inc. of San Diego, CA.
